This project, with the support of The Paul G. Allen Family Foundation, will further explore how human blood cells – B-cells – mature from their beginnings in our bone marrow to those that protect us from infection. It will focus on scanning and mapping the B-cell development process and on how deviations can result in cancers like leukemia and multiple myeloma. By mapping this process at a high resolution, we will develop a protocol to identify markers and rules for defining cell types and their relationships, and in doing so further the understanding of human wellness and disease.